antibodies against tgf β  (Boster Bio)

Journal: Frontiers in Microbiology

Article Title: Gut microbiota dysbiosis impairs TGF-β/Smad4 signaling to drive postoperative metastasis in colorectal cancer

doi: 10.3389/fmicb.2025.1654227

Figure Lengend Snippet: Effects of FMT on TGF-β/Smad4 signaling in an orthotopic CRC mouse model. (A) TGF-β and IL-10 concentration of four groups in serum ( n = 4 per group); (B) TGF-β concentration in liver tissue homogenates; (C–E) relative protein levels of TGF-β and Smad4 in liver tissue of four groups by western blot ( n = 3 per group); values are expressed as mean ± SEM. The Kruskal–Wallis test followed by Dunn’s post-hoc test was used for non-parametric data. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: After 2 h blocking with 5% BSA, membranes were probed overnight at 4 °C with primary antibodies against TGF-β (1:1,000; Boster, China) and Smad4 (1:1,000; Boster, China).

Techniques: Concentration Assay, Western Blot

Journal: Chinese Medicine

Article Title: Senkyunolide I suppresses hepatic stellate cell activation and liver fibrosis by reprogramming VDR-dependent fatty acid metabolism

doi: 10.1186/s13020-025-01133-x

Figure Lengend Snippet: VDR deficiency exacerbated hepatic fibrosis and steatosis in mice. A mRNA levels of fibrogenic markers ( α-Sma , Col1a1 , and Timp1 ). B mRNA levels of lipogenic enzymes ( Fasn , Acc-1 , and Acly ) and fatty acid desaturases ( Fads1/2 and Scd1 ) (n = 5). C Western blot analysis of VDR and TGFβ/Smad pathway (n = 3), D Serum levels of liver function markers (AST, ALT, ALP, and TBIL) (n = 5). * P < 0.05, ** P < 0.01

Article Snippet: Membranes were blocked with 5% BSA and probed overnight at 4 °C with antibodies against: α-SMA (1:2000), COL1 A1 (1:1000), FASN (10624-2-AP, Proteintech, 1:1000), VDR (AF8159, Affinity, 1:1000), p-Smad3 (1:2000), Smad3 (AF6362, Affinity, 1:2000), TGF-β1 (1:3000) and β-actin (BM3873, Boster, 1:2000).

Techniques: Western Blot

Journal: Chinese Medicine

Article Title: Senkyunolide I suppresses hepatic stellate cell activation and liver fibrosis by reprogramming VDR-dependent fatty acid metabolism

doi: 10.1186/s13020-025-01133-x

Figure Lengend Snippet: VDR activation attenuated TGF-β1-induced fibrosteatotic changes in HSCs. A Western blot analysis of VDR and α-SMA (n = 3). B mRNA levels of fibrogenic markers ( α-Sma , Col1a1 , and Timp1 ). C mRNA levels of lipogenic enzymes ( Fasn , Acc-1 , and Acly ) and fatty acid desaturases ( Fads1/2 and Scd1 ) (n = 6). * P < 0.05, ** P < 0.01

Article Snippet: Membranes were blocked with 5% BSA and probed overnight at 4 °C with antibodies against: α-SMA (1:2000), COL1 A1 (1:1000), FASN (10624-2-AP, Proteintech, 1:1000), VDR (AF8159, Affinity, 1:1000), p-Smad3 (1:2000), Smad3 (AF6362, Affinity, 1:2000), TGF-β1 (1:3000) and β-actin (BM3873, Boster, 1:2000).

Techniques: Activation Assay, Western Blot

Journal: Chinese Medicine

Article Title: Senkyunolide I suppresses hepatic stellate cell activation and liver fibrosis by reprogramming VDR-dependent fatty acid metabolism

doi: 10.1186/s13020-025-01133-x

Figure Lengend Snippet: SI inhibited TGF-β1-induced activation of HSCs. A Chemical structure of the SI. B Cell viability of HSCs. C mRNA levels of fibrogenic markers ( α-Sma , Col1a1 , and Timp1 ). D Immunofluorescence analysis of α-SMA. scale bar = 20 μm. (n = 6). E Western blot analysis of α-SMA. F Molecular docking results of VDR and SI. G CETSA analysis. (n = 3) * P < 0.05, ** P < 0.01

Article Snippet: Membranes were blocked with 5% BSA and probed overnight at 4 °C with antibodies against: α-SMA (1:2000), COL1 A1 (1:1000), FASN (10624-2-AP, Proteintech, 1:1000), VDR (AF8159, Affinity, 1:1000), p-Smad3 (1:2000), Smad3 (AF6362, Affinity, 1:2000), TGF-β1 (1:3000) and β-actin (BM3873, Boster, 1:2000).

Techniques: Activation Assay, Immunofluorescence, Western Blot

Journal: Chinese Medicine

Article Title: Senkyunolide I suppresses hepatic stellate cell activation and liver fibrosis by reprogramming VDR-dependent fatty acid metabolism

doi: 10.1186/s13020-025-01133-x

Figure Lengend Snippet: SI regulated VDR and fatty acid metabolism to suppress TGFβ/Smad pathway in CCl 4 -induced mice. A Immunohistochemistry analysis of TGF-β1, p-Smad3, α-SMA, COL1 A1. scale bar = 200 μm. (n = 5). B Western blot analysis of VDR, TGF-β1, and α-SMA. C mRNA levels of Cpt1a . D mRNA levels of lipogenic enzymes ( Fasn , Acc-1 , and Acly ) and fatty acid desaturases ( Fads1/2 and Scd1 ) (n = 6) * P < 0.05, ** P < 0.01

Article Snippet: Membranes were blocked with 5% BSA and probed overnight at 4 °C with antibodies against: α-SMA (1:2000), COL1 A1 (1:1000), FASN (10624-2-AP, Proteintech, 1:1000), VDR (AF8159, Affinity, 1:1000), p-Smad3 (1:2000), Smad3 (AF6362, Affinity, 1:2000), TGF-β1 (1:3000) and β-actin (BM3873, Boster, 1:2000).

Techniques: Immunohistochemistry, Western Blot

Journal: Chinese Medicine

Article Title: Senkyunolide I suppresses hepatic stellate cell activation and liver fibrosis by reprogramming VDR-dependent fatty acid metabolism

doi: 10.1186/s13020-025-01133-x

Figure Lengend Snippet: SI inhibited lipid accumulation through regulating fatty acid metabolism in TGF-β1-induced HSCs. A lipid droplet accumulation assessed by Nile Red and CT-8. scale bar = 20 μm. (n = 6). B Cpt1a expression. C Western blot analysis of FASN. (n = 3). D mRNA levels of lipogenic enzymes ( Fasn , Acc-1 , and Acly ) and fatty acid desaturases ( Fads1/2 and Scd1 ) (n = 6) * P < 0.05, ** P < 0.01

Article Snippet: Membranes were blocked with 5% BSA and probed overnight at 4 °C with antibodies against: α-SMA (1:2000), COL1 A1 (1:1000), FASN (10624-2-AP, Proteintech, 1:1000), VDR (AF8159, Affinity, 1:1000), p-Smad3 (1:2000), Smad3 (AF6362, Affinity, 1:2000), TGF-β1 (1:3000) and β-actin (BM3873, Boster, 1:2000).

Techniques: Expressing, Western Blot

Journal: Chinese Medicine

Article Title: Senkyunolide I suppresses hepatic stellate cell activation and liver fibrosis by reprogramming VDR-dependent fatty acid metabolism

doi: 10.1186/s13020-025-01133-x

Figure Lengend Snippet: VDR knockout abolished the SI-induced inhibition of HSCs activation. A Western blot analysis (n = 3). B Immunofluorescence analysis of α-SMA. scale bar = 20 μm. (n = 6). C mRNA levels of fibrogenic markers ( α-Sma , Col1a1 , and Timp1 ). (n = 6) *P < 0.05, **P < 0.01 compared with si-NC + TGF-β1; $ P < 0.05, $$ P < 0.01 compared with si-NC; # P < 0.05, ## P < 0.01 compared with si-VDR + TGF-β1

Article Snippet: Membranes were blocked with 5% BSA and probed overnight at 4 °C with antibodies against: α-SMA (1:2000), COL1 A1 (1:1000), FASN (10624-2-AP, Proteintech, 1:1000), VDR (AF8159, Affinity, 1:1000), p-Smad3 (1:2000), Smad3 (AF6362, Affinity, 1:2000), TGF-β1 (1:3000) and β-actin (BM3873, Boster, 1:2000).

Techniques: Knock-Out, Inhibition, Activation Assay, Western Blot, Immunofluorescence

Journal: Chinese Medicine

Article Title: Senkyunolide I suppresses hepatic stellate cell activation and liver fibrosis by reprogramming VDR-dependent fatty acid metabolism

doi: 10.1186/s13020-025-01133-x

Figure Lengend Snippet: SI reprogramed fatty acid metabolism through activating VDR in TGFβ1-induced HSCs. A Lipid droplet accumulation. scale bar = 20 μm. B Cpt1a expression. C Western blot analysis of FASN. (n = 3). D mRNA levels of lipogenic enzymes ( Fasn , Acc-1 , and Acly ) and fatty acid desaturases ( Fads1/2 and Scd1 ). (n = 6) * P < 0.05, ** P < 0.01. * P < 0.05, ** P < 0.01 compared with si-NC + TGF-β1; $ P < 0.05, $$ P < 0.01 compared with si-NC; # P < 0.05, ## P < 0.01 compared with si-VDR + TGF-β1

Article Snippet: Membranes were blocked with 5% BSA and probed overnight at 4 °C with antibodies against: α-SMA (1:2000), COL1 A1 (1:1000), FASN (10624-2-AP, Proteintech, 1:1000), VDR (AF8159, Affinity, 1:1000), p-Smad3 (1:2000), Smad3 (AF6362, Affinity, 1:2000), TGF-β1 (1:3000) and β-actin (BM3873, Boster, 1:2000).

Techniques: Expressing, Western Blot